| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| Cj1329 | gne | Cj1329 | Cj1131c | Sugar-phosphate nucleotide transferase | UDP-GlcNAc/Glc 4-epimerase | 0.517 |
| Cj1329 | pglC | Cj1329 | Cj1124c | Sugar-phosphate nucleotide transferase | Galactosyltransferase; Glycosyl-1-phosphate transferase that mediates the first step in the biosynthesis of the undecaprenyl-linked heptasaccharide donor in the N-linked protein glycosylation pathway. Catalyzes the linking of uridine 5’- diphosphobacillosamine (UDP-Bac) to undecaprenyl phosphate to create the first membrane-associated intermediate undecaprenylpyrophosphate-linked Bac (Und-PP-Bac) | 0.475 |
| Cj1329 | pglD | Cj1329 | Cj1123c | Sugar-phosphate nucleotide transferase | Acetyltransferase; Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N’-diacetylbacillosamine in the N-linked protein glycosylation pathway | 0.468 |
| Cj1329 | pglE | Cj1329 | Cj1121c | Sugar-phosphate nucleotide transferase | UDP-4-keto-6-deoxy-GlcNAc C4 aminotransferase; Aminotransferase involved in the bacillosamine biosynthesis pathway by producing UDP-4-amino-4,6-dideoxy-alpha-D- GlcNAc (UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D- glucopyranose), a precursor used in the production of the glycan component 2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose. Required for host colonization and virulence. Involved in the N- linked protein glycosylation pathway | 0.547 |
| Cj1329 | pglF | Cj1329 | Cj1120c | Sugar-phosphate nucleotide transferase | UDP-GlcNAc C4,6 dehydratase; C6 dehydratase involved in the bacillosamine biosynthesis pathway by generating UDP-2-acetamido-2,6-dideoxy- alpha-D-xylo-4-hexulose from UDP-N-acetyl-alpha-D-glucosamine. Involved in the N-linked protein glycosylation pathway | 0.457 |
| Cj1329 | pglH | Cj1329 | Cj1129c | Sugar-phosphate nucleotide transferase | GalNAc transferase/polymerase; Transfers 3 terminal GalNAc residues to the carrier polyisoprene in the N-linked protein glycosylation pathway. Acts as a polymerase via a processive reaction in which intermediates do not dissociate from the enzyme and by using a single active site for multiple GalNAc transfers. Uses product inhibition to stop the enzyme from carrying out more than 3 GalNAc transfer reactions | 0.987 |
| Cj1329 | pglI | Cj1329 | Cj1128c | Sugar-phosphate nucleotide transferase | Glucosyl transferase; Glucosyltransferase that adds he final branching glucose to complete the final heptasaccharide structure in the N-linked protein glycosylation pathway | 0.855 |
| Cj1329 | pglK | Cj1329 | Cj1130c | Sugar-phosphate nucleotide transferase | Flippase; Mediates the ATP-dependent translocation of the undecaprenylpyrophosphate-linked heptasaccharide intermediate across the cell membrane; this is an essential step during the N- linked protein glycosylation pathway. Transport across the membrane is effected via ATP-driven conformation changes. Most likely, only the polar and charged part of the glycolipid enter the substrate-binding cavity, and the lipid tail remains exposed to the membrane lipids during the transmembrane flipping process | 0.749 |
| gne | Cj1329 | Cj1131c | Cj1329 | UDP-GlcNAc/Glc 4-epimerase | Sugar-phosphate nucleotide transferase | 0.517 |
| gne | pglA | Cj1131c | Cj1125c | UDP-GlcNAc/Glc 4-epimerase | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | 0.972 |
| gne | pglB | Cj1131c | Cj1126c | UDP-GlcNAc/Glc 4-epimerase | Oligosaccharide transferase to N-glycosylate proteins; Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins | 0.895 |
| gne | pglC | Cj1131c | Cj1124c | UDP-GlcNAc/Glc 4-epimerase | Galactosyltransferase; Glycosyl-1-phosphate transferase that mediates the first step in the biosynthesis of the undecaprenyl-linked heptasaccharide donor in the N-linked protein glycosylation pathway. Catalyzes the linking of uridine 5’- diphosphobacillosamine (UDP-Bac) to undecaprenyl phosphate to create the first membrane-associated intermediate undecaprenylpyrophosphate-linked Bac (Und-PP-Bac) | 0.914 |
| gne | pglD | Cj1131c | Cj1123c | UDP-GlcNAc/Glc 4-epimerase | Acetyltransferase; Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N’-diacetylbacillosamine in the N-linked protein glycosylation pathway | 0.948 |
| gne | pglE | Cj1131c | Cj1121c | UDP-GlcNAc/Glc 4-epimerase | UDP-4-keto-6-deoxy-GlcNAc C4 aminotransferase; Aminotransferase involved in the bacillosamine biosynthesis pathway by producing UDP-4-amino-4,6-dideoxy-alpha-D- GlcNAc (UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D- glucopyranose), a precursor used in the production of the glycan component 2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose. Required for host colonization and virulence. Involved in the N- linked protein glycosylation pathway | 0.901 |
| gne | pglF | Cj1131c | Cj1120c | UDP-GlcNAc/Glc 4-epimerase | UDP-GlcNAc C4,6 dehydratase; C6 dehydratase involved in the bacillosamine biosynthesis pathway by generating UDP-2-acetamido-2,6-dideoxy- alpha-D-xylo-4-hexulose from UDP-N-acetyl-alpha-D-glucosamine. Involved in the N-linked protein glycosylation pathway | 0.963 |
| gne | pglH | Cj1131c | Cj1129c | UDP-GlcNAc/Glc 4-epimerase | GalNAc transferase/polymerase; Transfers 3 terminal GalNAc residues to the carrier polyisoprene in the N-linked protein glycosylation pathway. Acts as a polymerase via a processive reaction in which intermediates do not dissociate from the enzyme and by using a single active site for multiple GalNAc transfers. Uses product inhibition to stop the enzyme from carrying out more than 3 GalNAc transfer reactions | 0.979 |
| gne | pglI | Cj1131c | Cj1128c | UDP-GlcNAc/Glc 4-epimerase | Glucosyl transferase; Glucosyltransferase that adds he final branching glucose to complete the final heptasaccharide structure in the N-linked protein glycosylation pathway | 0.967 |
| gne | pglK | Cj1131c | Cj1130c | UDP-GlcNAc/Glc 4-epimerase | Flippase; Mediates the ATP-dependent translocation of the undecaprenylpyrophosphate-linked heptasaccharide intermediate across the cell membrane; this is an essential step during the N- linked protein glycosylation pathway. Transport across the membrane is effected via ATP-driven conformation changes. Most likely, only the polar and charged part of the glycolipid enter the substrate-binding cavity, and the lipid tail remains exposed to the membrane lipids during the transmembrane flipping process | 0.967 |
| pglA | gne | Cj1125c | Cj1131c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | UDP-GlcNAc/Glc 4-epimerase | 0.972 |
| pglA | pglB | Cj1125c | Cj1126c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | Oligosaccharide transferase to N-glycosylate proteins; Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins | 0.960 |
