| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| gne | pglA | Cj1131c | Cj1125c | UDP-GlcNAc/Glc 4-epimerase | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | 0.972 |
| gne | pglB | Cj1131c | Cj1126c | UDP-GlcNAc/Glc 4-epimerase | Oligosaccharide transferase to N-glycosylate proteins; Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins | 0.895 |
| gne | pglC | Cj1131c | Cj1124c | UDP-GlcNAc/Glc 4-epimerase | Galactosyltransferase; Glycosyl-1-phosphate transferase that mediates the first step in the biosynthesis of the undecaprenyl-linked heptasaccharide donor in the N-linked protein glycosylation pathway. Catalyzes the linking of uridine 5’- diphosphobacillosamine (UDP-Bac) to undecaprenyl phosphate to create the first membrane-associated intermediate undecaprenylpyrophosphate-linked Bac (Und-PP-Bac) | 0.914 |
| gne | pglD | Cj1131c | Cj1123c | UDP-GlcNAc/Glc 4-epimerase | Acetyltransferase; Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N’-diacetylbacillosamine in the N-linked protein glycosylation pathway | 0.948 |
| gne | pglF | Cj1131c | Cj1120c | UDP-GlcNAc/Glc 4-epimerase | UDP-GlcNAc C4,6 dehydratase; C6 dehydratase involved in the bacillosamine biosynthesis pathway by generating UDP-2-acetamido-2,6-dideoxy- alpha-D-xylo-4-hexulose from UDP-N-acetyl-alpha-D-glucosamine. Involved in the N-linked protein glycosylation pathway | 0.963 |
| gne | pglH | Cj1131c | Cj1129c | UDP-GlcNAc/Glc 4-epimerase | GalNAc transferase/polymerase; Transfers 3 terminal GalNAc residues to the carrier polyisoprene in the N-linked protein glycosylation pathway. Acts as a polymerase via a processive reaction in which intermediates do not dissociate from the enzyme and by using a single active site for multiple GalNAc transfers. Uses product inhibition to stop the enzyme from carrying out more than 3 GalNAc transfer reactions | 0.979 |
| gne | pglI | Cj1131c | Cj1128c | UDP-GlcNAc/Glc 4-epimerase | Glucosyl transferase; Glucosyltransferase that adds he final branching glucose to complete the final heptasaccharide structure in the N-linked protein glycosylation pathway | 0.967 |
| gne | pglJ | Cj1131c | Cj1127c | UDP-GlcNAc/Glc 4-epimerase | GalNAc transferase; Adds a GalNAc residue on to the Und-PP-Bac2,4diNAc- GalNAc disaccharide in the N-linked protein glycosylation pathway. Transfers the third sugar in the heptasaccharide biosynthesis | 0.980 |
| gne | pglK | Cj1131c | Cj1130c | UDP-GlcNAc/Glc 4-epimerase | Flippase; Mediates the ATP-dependent translocation of the undecaprenylpyrophosphate-linked heptasaccharide intermediate across the cell membrane; this is an essential step during the N- linked protein glycosylation pathway. Transport across the membrane is effected via ATP-driven conformation changes. Most likely, only the polar and charged part of the glycolipid enter the substrate-binding cavity, and the lipid tail remains exposed to the membrane lipids during the transmembrane flipping process | 0.967 |
| kpsF | pglH | Cj1443c | Cj1129c | D-arabinose 5-phosphate isomerase | GalNAc transferase/polymerase; Transfers 3 terminal GalNAc residues to the carrier polyisoprene in the N-linked protein glycosylation pathway. Acts as a polymerase via a processive reaction in which intermediates do not dissociate from the enzyme and by using a single active site for multiple GalNAc transfers. Uses product inhibition to stop the enzyme from carrying out more than 3 GalNAc transfer reactions | 0.608 |
| kpsF | pglJ | Cj1443c | Cj1127c | D-arabinose 5-phosphate isomerase | GalNAc transferase; Adds a GalNAc residue on to the Und-PP-Bac2,4diNAc- GalNAc disaccharide in the N-linked protein glycosylation pathway. Transfers the third sugar in the heptasaccharide biosynthesis | 0.608 |
| kpsF | pglK | Cj1443c | Cj1130c | D-arabinose 5-phosphate isomerase | Flippase; Mediates the ATP-dependent translocation of the undecaprenylpyrophosphate-linked heptasaccharide intermediate across the cell membrane; this is an essential step during the N- linked protein glycosylation pathway. Transport across the membrane is effected via ATP-driven conformation changes. Most likely, only the polar and charged part of the glycolipid enter the substrate-binding cavity, and the lipid tail remains exposed to the membrane lipids during the transmembrane flipping process | 0.917 |
| pglA | gne | Cj1125c | Cj1131c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | UDP-GlcNAc/Glc 4-epimerase | 0.972 |
| pglA | pglB | Cj1125c | Cj1126c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | Oligosaccharide transferase to N-glycosylate proteins; Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins | 0.960 |
| pglA | pglC | Cj1125c | Cj1124c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | Galactosyltransferase; Glycosyl-1-phosphate transferase that mediates the first step in the biosynthesis of the undecaprenyl-linked heptasaccharide donor in the N-linked protein glycosylation pathway. Catalyzes the linking of uridine 5’- diphosphobacillosamine (UDP-Bac) to undecaprenyl phosphate to create the first membrane-associated intermediate undecaprenylpyrophosphate-linked Bac (Und-PP-Bac) | 0.996 |
| pglA | pglD | Cj1125c | Cj1123c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | Acetyltransferase; Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N’-diacetylbacillosamine in the N-linked protein glycosylation pathway | 0.979 |
| pglA | pglF | Cj1125c | Cj1120c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | UDP-GlcNAc C4,6 dehydratase; C6 dehydratase involved in the bacillosamine biosynthesis pathway by generating UDP-2-acetamido-2,6-dideoxy- alpha-D-xylo-4-hexulose from UDP-N-acetyl-alpha-D-glucosamine. Involved in the N-linked protein glycosylation pathway | 0.956 |
| pglA | pglH | Cj1125c | Cj1129c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | GalNAc transferase/polymerase; Transfers 3 terminal GalNAc residues to the carrier polyisoprene in the N-linked protein glycosylation pathway. Acts as a polymerase via a processive reaction in which intermediates do not dissociate from the enzyme and by using a single active site for multiple GalNAc transfers. Uses product inhibition to stop the enzyme from carrying out more than 3 GalNAc transfer reactions | 0.963 |
| pglA | pglI | Cj1125c | Cj1128c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | Glucosyl transferase; Glucosyltransferase that adds he final branching glucose to complete the final heptasaccharide structure in the N-linked protein glycosylation pathway | 0.979 |
| pglA | pglJ | Cj1125c | Cj1127c | GalNAc transferase; Adds the first GalNAc residue on to the isoprenoid- linked bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose) carrier in the N-linked protein glycosylation pathway. Acts first on the undecaprenylpyrophosphate-linked bacillosamine (Und-PP-Bac) substrate to yield the disaccharide | GalNAc transferase; Adds a GalNAc residue on to the Und-PP-Bac2,4diNAc- GalNAc disaccharide in the N-linked protein glycosylation pathway. Transfers the third sugar in the heptasaccharide biosynthesis | 0.993 |
